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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Omega-3 polyunsaturated fatty acid attenuates the inflammatory response by modulating microglia polarization through SIRT1-mediated deacetylation of the HMGB1/NF-κB pathway following experimental traumatic brain injury

Fig. 7

ω-3 PUFA supplementation suppressed HMGB1 pathway via elevating SIRT1 activity in lesioned cortices 3 days after TBI. a Co-IP analysis showed that a reduction in HMGB1 acetylation following ω-3 PUFA supplementation compared with the TBI group (p < 0.05). b Co-IP analysis showed a reduction in NF-κB acetylation following ω-3 PUFA supplementation compared with the TBI group (p < 0.05). c HMGB1 acetylation caused it to dissociate from SIRT1, thereby promoting HMGB1 extracellular secretion after TBI; ω-3 PUFA supplementation also induced direct interactions between SIRT1 and HMGB1 (p < 0.05). d The inhibitory effect of ω-3 PUFA supplementation on the neuroinflammatory response was reversed by pharmacological inhibition of SIRT1. e The inhibitory effect of ω-3 PUFA supplementation on HMGB1/NF-κB pathway activation was reversed by pharmacological inhibition of SIRT1. Values are expressed as mean ± standard deviation (n = 6 per group). N.S., p > 0.05, *p < 0.05, **p < 0.01. Scale bars = 50 μm

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Journal of Neuroinflammation

ISSN: 1742-2094