Fig. 1From: Mesenchymal stem cells attenuate blood-brain barrier leakage after cerebral ischemia in miceBone-derived mesenchymal stem cell (MSC) isolation, identification, and injection. a Morphology of MSCs in cell culture. Cultured cells showed typically spindle-shaped morphology under phase-contrast microscopy. b Schematic diagram of cell injection into the striatum of the brain and the survival of MSCs after injection. c Green fluorescent (CFDA SE dye) cells were located in the ischemic hemisphere after 3 days of injection. Scale bar = 300 μm. d Cytometry analysis depicted that 99.99+% cultured cells were positive for CD29 and CD90 and negative for CD31 and CD45Back to article page