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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Acute dose of melatonin via Nrf2 dependently prevents acute ethanol-induced neurotoxicity in the developing rodent brain

Fig. 1

Schematic representation of the in vivo study design. Randomly selected male and female Sprague-Dawley postnatal day 7 rat pups of average body weight (18 g) were used (n = 15 pups/group). The postnatal rats were divided into the following groups: (1) Rat pups treated with a single intraperitoneal (I.P.) injection of saline as a vehicle, grouped as control (C). (2) Rat pups treated with a single dose of ethanol (E) (5 g/kg, subcutaneous (S.C.)), grouped as E. (3) Rat pups co-treated with a single dose of ethanol (5 g/kg, (S.C.)) and a single dose of melatonin (20 mg/kg, I.P.), grouped as E + M. (4) Rat pups treated with a single dose of melatonin (M) 20 mg/kg, I.P., grouped as M. Four hours after a single S.C. and I.P. injections, all of the rat pups were sacrificed for further biochemical and immunohistochemical analyses

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