Skip to main content
Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Acute dose of melatonin via Nrf2 dependently prevents acute ethanol-induced neurotoxicity in the developing rodent brain

Fig. 4

Acute melatonin prevents the acute ethanol-induced activation of the MAPK p-P38/p-JNK pathway in the rat pups and in HT22 cells that were exposed to ethanol. a Western blot results of p-P38, total P-38, p-JNK, and total JNK in the rat pups. The bands were quantified using Sigma Gel software, and the differences are presented in a histogram. β-Actin was used as a loading control. n = 10 pups/group, and the number of experiments = 3. b, c Representative images of the co-localized immunofluorescence reactivity of p-P38 and p-JNK in the cortices and CA1 regions of the hippocampi in the rat pups. n = 5 pups/group, and the number of experiments = 3. Magnification × 40. Scale bar = 50 μm. d Western blots and the densitometric analysis of p-P38, total P-38, p-JNK, and total JNK expression in the HT22 cells that were subjected to Nrf2 siRNA and treated with ethanol (100 mM) and melatonin (100 μM) for 12 h. β-Actin was used as a loading control. The data are expressed as the mean ± SEM, and the number of experiments = 3. The data are presented relative to control values. Significance = P < 0.05. σ Significantly different from the control saline-treated rat pups; Φ significantly different from the ethanol-treated rat pups. Similarly for in vitro studies, σ significantly different from the non-treated HT22 cells, Φ significantly different from the ethanol-exposed HT22 cells, and ω significantly different from the ethanol+melatonin-exposed HT22 cells

Back to article page