Fig. 5

IL-1β impaired cLTP-induced signaling associated with actin dynamics. a Western blots of G-actin and F-actin. b Quantification of the blots shown in a. Data are mean ± SEM from three independent experiments expressed in terms of the ratio of G-actin to F-actin obtained in the control cultures (*p < 0.05, ANOVA). c Western blots of phosphorylation of cofilin (P-Cof). d Quantification of the blots shown in c. Data are means ± SE of values from three independent experiments expressed in terms of P-Cof obtained in the control cultures (*p < 0.05, ANOVA). e Representative fluorescence images show the expression of sGluA1 measured by immunostaining in cells treated with cLTP in the presence or absence of IL-1β with or without Ser3 peptide. Scale bar, 5 μm. f Quantification of the images shown in e. Data are mean ± SEM from three independent experiments expressed in terms of control (*p < 0.05, ANOVA)