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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: IL-1β suppresses cLTP-induced surface expression of GluA1 and actin polymerization via ceramide-mediated Src activation

Fig. 6

Neutral sphingomyelinase inhibitor GW4869 attenuated IL-1β-mediated suppression of cLTP-induced synaptic plasticity. a Representative fluorescence images show F-actin, which was stained by phallodin, in unstimulated control cells and cells treated with cLTP in the presence or absence of IL-1β with or without GW4869 (5 μM). Scale bar, 10 μm. b Quantification of the images shown in a. The intensity of phallodin staining was normalized by MAP2 staining. Data are mean ± SEM from three independent experiments expressed in terms of control (*p < 0.05, ANOVA). c Neutral sphingomyelinase inhibitor GW4869 (5 μM) attenuated IL-1β-mediated suppression of cLTP-induced GluA1 insertion. Representative fluorescence images show the expression of sGluA1 in unstimulated control cells and cells treated with cLTP in the presence or absence of IL-1β with or without GW4869. Scale bar, 10 μm. d Quantification of the images shown in c. Data are mean ± SEM from three independent experiments expressed in terms of control (*p < 0.05, ANOVA). e Representative fluorescence images show the expression of sGluA1 in unstimulated control cells and cells treated with cLTP in the presence or absence of C2-ceramide (10 μM). Scale bar, 20 μm. f Quantification of the images shown in e. Data are mean ± SEM from three independent experiments expressed in terms of control (*p < 0.05, ANOVA)

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