Fig. 2

Decreased Ng-CaM interaction reduces the expressions of synaptic plasticity markers in HAND patients. a IHC staining of CaM in the FC tissues from control, HAND (−), and HAND (+) subjects were performed. Scale bar indicates 50 μm. b Mean area/cell and mean intensity/cell were calculated from five different fields from each subject using the NIS Elements software. c Lysates prepared from control, HAND (−), and HAND (+) FC tissues were subjected to co-IP using anti-Ng antibody. Western blot was performed using anti-CaM antibody. Each lane represents sample from a single donor. Input represents presence of CaM in samples used for co-IP. d Control, HAND (−), and HAND (+) FC tissues were lysed, and 5 μg of each lysate was analyzed by western blot using antibodies against CaMKII, CREB, Syp, Syn I, and MAP2. e Relative band intensities were normalized with MAP2. Densitometry quantification of western blot data represents ± SEM of four independent observations. *p < 0.05 compared to control. NS not significant