Gene | Primer sequences | Annealing temperature (°C) |
---|---|---|
Interleukin-1-β (IL-1β) | F: 5′-CCTCCTTGCCTCTGATGG-3′ R: 5′-AGTGCTGCCTAATGTCCC-3′ | 60 |
Tumor necrosis factor (TNF-α) | F: 5′-CCCCAGTCTGTATCCTTCT-3′ R: 5′-ACTGTCCCAGCATCTTGT-3′ | 59 |
Interleukin-6 (IL-6) | F: 5′-GGCAATTCTGATTGTATG-3′ R: 5′-CTCTGGCTTTGTCTTTCT-3′ | 56 |
Interleukin-8 (IL-8) | F: 5′-TGCCGTGACCTCAAGATGTGCC-3′ R: 5′-CATCCACAAGCGTGCTGTAGGTG-3′ | 60 |
Interleukin-10 (IL-10) | F: 5′-CCAAGCCTTATCGGAAATGA-3′ R: 5′-TTCTCACCCAGGGAATTCAA-3′ | 60 |
Glyceraldehye-3-phosphate dehydrogenase (GAPDH) | F: 5′-ATTCAACGGCACAGTCAA-3′ R: 5′-CTCGCTCCTGGAAGATGG-3′ | 56 |