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Table 1 PCR conditions for inflammatory cytokines

From: Evidence of the impact of systemic inflammation on neuroinflammation from a non-bacterial endotoxin animal model

Gene Primer sequences Annealing temperature (°C)
Interleukin-1-β (IL-1β) F: 5′-CCTCCTTGCCTCTGATGG-3′
R: 5′-AGTGCTGCCTAATGTCCC-3′
60
Tumor necrosis factor (TNF-α) F: 5′-CCCCAGTCTGTATCCTTCT-3′
R: 5′-ACTGTCCCAGCATCTTGT-3′
59
Interleukin-6 (IL-6) F: 5′-GGCAATTCTGATTGTATG-3′
R: 5′-CTCTGGCTTTGTCTTTCT-3′
56
Interleukin-8 (IL-8) F: 5′-TGCCGTGACCTCAAGATGTGCC-3′
R: 5′-CATCCACAAGCGTGCTGTAGGTG-3′
60
Interleukin-10 (IL-10) F: 5′-CCAAGCCTTATCGGAAATGA-3′
R: 5′-TTCTCACCCAGGGAATTCAA-3′
60
Glyceraldehye-3-phosphate dehydrogenase (GAPDH) F: 5′-ATTCAACGGCACAGTCAA-3′
R: 5′-CTCGCTCCTGGAAGATGG-3′
56