Table 2 Primary antibodies used in Western blot analysis
Phosphor peptide/kinase | Dilution | Resource | Catalog no. |
|---|---|---|---|
AT8 (pSer202/Thr205) | 1:1000 | Thermo Fisher Scientific, USA | MN1020 |
AT180 (pThr231/Ser235) | 1:1000 | Thermo Fisher Scientific, USA | MN1040 |
pS404 (pSer404) | 1:3000 | Biosource, USA | 44-758G |
Total tau (polyclonal rabbit anti-human tau) | 1:30,000 | DAKO, Denmark | A0024 |
Jak2 | 1:1000 | Cell Signaling Technology | 3230S |
phospho-Jak2 (Tyr1007/1008) | 1:1000 | Cell Signaling Technology | 3771S |
Stat3 | 1:1000 | Cell Signaling Technology | 9139S |
Phospho-Stat3 (Tyr705) | 1:1000 | Cell Signaling Technology | 9131S |
Glycogen synthase kinase-3β (GSK-3β) | 1:1000 | Cell Signaling Technology | 9315S |
Phospho-GSK-3β (Ser9) | 1:1000 | Cell Signaling Technology | 9336S |
Extracellular signal-regulated kinase (ERK) 1/2 | 1:3000 | Cell Signaling Technology | 9102 |
Phospho-ERK1/2 (Thr202/Tyr204) | 1:3000 | Cell Signaling Technology | 9101S |
Stress-activated protein kinases (SAPK)/c-Jun N-terminal kinase (JNK) | 1:3000 | Cell Signaling Technology | 9258S |
Phospho-SAPK-JNK (Thr183/Tyr185) | 1:1000 | Cell Signaling Technology | 9251S |
PP2A-C | 1:3000 | Millipore, USA | 05-421 |
Phospho-PP2A (Tyr307) | 1:3000 | Epitomics | 1155-1 |
β-actin | 1:30,000 | Sigma-Aldrich | A5441 |
Glyceraldehye-3-phosphate dehydrogenase (GAPDH) | 1:3000 | Sigma-Aldrich | G8795 |
α-Tubulin | 1:40,000 | Sigma-Aldrich | T9026 |