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Table 2 Primary antibodies used in Western blot analysis

From: Evidence of the impact of systemic inflammation on neuroinflammation from a non-bacterial endotoxin animal model

Phosphor peptide/kinase Dilution Resource Catalog no.
AT8 (pSer202/Thr205) 1:1000 Thermo Fisher Scientific, USA MN1020
AT180 (pThr231/Ser235) 1:1000 Thermo Fisher Scientific, USA MN1040
pS404 (pSer404) 1:3000 Biosource, USA 44-758G
Total tau (polyclonal rabbit anti-human tau) 1:30,000 DAKO, Denmark A0024
Jak2 1:1000 Cell Signaling Technology 3230S
phospho-Jak2 (Tyr1007/1008) 1:1000 Cell Signaling Technology 3771S
Stat3 1:1000 Cell Signaling Technology 9139S
Phospho-Stat3 (Tyr705) 1:1000 Cell Signaling Technology 9131S
Glycogen synthase kinase-3β (GSK-3β) 1:1000 Cell Signaling Technology 9315S
Phospho-GSK-3β (Ser9) 1:1000 Cell Signaling Technology 9336S
Extracellular signal-regulated kinase (ERK) 1/2 1:3000 Cell Signaling Technology 9102
Phospho-ERK1/2 (Thr202/Tyr204) 1:3000 Cell Signaling Technology 9101S
Stress-activated protein kinases (SAPK)/c-Jun N-terminal kinase (JNK) 1:3000 Cell Signaling Technology 9258S
Phospho-SAPK-JNK (Thr183/Tyr185) 1:1000 Cell Signaling Technology 9251S
PP2A-C 1:3000 Millipore, USA 05-421
Phospho-PP2A (Tyr307) 1:3000 Epitomics 1155-1
β-actin 1:30,000 Sigma-Aldrich A5441
Glyceraldehye-3-phosphate dehydrogenase (GAPDH) 1:3000 Sigma-Aldrich G8795
α-Tubulin 1:40,000 Sigma-Aldrich T9026