Fig. 5
TNF blockade limits ET-1 expression during progressive EAE. a Representative confocal images (Z-stack) of longitudinal spinal cord sections stained for ET-1 during chronic (d30) EAE in WT and GFAPγR1Δ mice, treated with isotype control or anti-TNF mAb. b Quantification of ET-1+ area per field (mm2) in spinal cords stained as indicated in panel a during acute (d19) and chronic (d30) EAE. Data are from six to eight separate fields from two individual mice per group and two independent experiments. c ET-1 mRNA levels in spinal cords from EAE mice as indicated determined by RT-PCR. Data represent mean ± SEM values from individual mice of two separate experiments, each comprising three to four mice per group and time point. d Representative confocal images (Z-stack) of spinal cords from WT and GFAPγR1Δ mice, treated with isotype control or anti-TNF mAb, during chronic (d30) EAE stained for ET-1 (green), fluoromyelin (red), and DAPI (blue) within demyelinated lesion (dotted line). Images representative of three to four separate fields from two to three mice per group. Scale bars, 50 μm. P values were determined by Student’s t test. GFAPγR1Δ in all panels represents GFAPγR1Δ mice treated with isotype control mAb