Fig. 5

Brain pericytes and endothelia have overlapping but distinct responses to IL-1β. Endothelia and pericytes were cultured and treated with IL-1β (10 ng/mL, 24 h) or vehicle before conditioned media were taken, and cytokine secretion measured using a Proteome Profiler™ Human XL Cytokine Array Kit. Representative cytokine array blots of conditioned media from a pericytes and b endothelia treated with either vehicle or IL-1β. c Scatterplot of the correlation between spot intensities from each repeat. d Non-biased clustering analysis of secretions in endothelia and pericytes (n = 2 cases), and e Venn diagram of secretion profile of endothelia and pericytes