Fig. 4

Restricted EGFP expression in microglial cells located in peri-plaque areas of the CB₂^EGFP/f/f/5xFAD mouse hippocampus. a–l Low-magnification photographs of EGFP⁺ microglial cells (a, b) in close association to beta-amyloid neuritic plaques (c, d). Medium- (e–h) and high-magnification (i–l) photographs of EGFP⁺ microglial cells. Detailed co-localization immunofluorescent analysis reveals a complete overlap between EGFP⁺ cells and Iba1⁺ cells, indicative of their macrophage/microglia nature, and a selective association to amyloid-enriched plaques (stained with methoxy-XO4). Scale bars, 100 μm (a–d), 25 μm (e–h), and 25 μm (i–l). SBC (subiculum)