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Table 2 Antibodies used for (A) Chipcytometry and (B) flow cytometry

From: Immunophenotyping of cerebrospinal fluid cells by Chipcytometry

Epitope

Clone

Company

Dilution

A

 CD3

UCHT1

BD

1:100

 CD4

RPA-T4

Biolegend

1:100

 CD8

RPA-T8

BD

1:30

 CD14

RMO52

Beckman Coulter

1:30

 CD16

3G8

Biolegend

1:100

 CD19

HIB 19

eBioscience

1:100

 CD24

ML5

Biolegend

1:300

 CD25

M-A251

BD

1:50

 CD27

LG.3A10

Biolegend

1:300

 CD38

HB7

BD

1:50

 CD45RA

HI100

Biolegend

1:400

 CD54

HA58

BD

1:30

 CD56

AF12-7H3

Miltenyi Biotec

1:30

 HLA-DR

G46-6

BD

1:50

 IgD

IA6-2

Biolegend

1:30

 IgM

MHM-88

Biolegend

1:250

 IgG

IS11-3B2.2.3

Miltenyi Biotec

1:100

 IgA

IS11-8E10

Miltenyi Biotec

1:200

 Kappa light chain

MHK-49

Biolegend

1:200

 Lambda light chain

MHL-38

Biolegend

1:200

B

 CD3 (PC 5.5)

UCHT1

Beckman Coulter

1:200

 CD4 (APC)

12B8.2

Beckman Coulter

1:200

 CD8 (pacific blue)

B9.11

Beckman Coulter

1:200

 CD14 (FITC)

RM052

Beckman Coulter

1:200

 CD19 (APC-A700)

J3-119

Beckman Coulter

1:200

 CD45 (krome orange)

J.33

Beckman Coulter

1:200

  1. All antibodies used for Chipcytometry were labeled with the fluorochrome phycoerythrin (PE). The fluorochromes used in flow cytometry are indicated in parentheses