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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: PM2.5 exposure aggravates oligomeric amyloid beta-induced neuronal injury and promotes NLRP3 inflammasome activation in an in vitro model of Alzheimer’s disease

Fig. 4

PM2.5 exposure increases ROS level in oAβ-stimulated microglia. Microglia with different treatment were incubated with CellROX. a Average staining intensity per unit area of stain quantified for three random fields of view. Microglia were treated with different stimulation, according to different groups. b The degree of oxidative stress (indicated by intracellular ROS level) was detected using a commercial detection kit. c The mitochondrial ROS level was detected by flow cytometry using MitoSOX Red superoxide indicator. d NADPH oxidase activity was tested by lucigenin-enhanced chemiluminescence. All figures are representative of three independent experiments, performed in triplicate. *P < 0.05 vs. LPS-primed microglia. #P < 0.05 vs. LPS-primed microglia stimulated with oAβ

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