Fig. 7From: PM2.5 exposure aggravates oligomeric amyloid beta-induced neuronal injury and promotes NLRP3 inflammasome activation in an in vitro model of Alzheimer’s diseaseROS and NLRP3 inflammasome activation is required for PM2.5-induced neuronal injury in neurons-microglia co-cultures. In a transwell co-culture system, neurons-microglia co-cultures were stimulated with oAβ for 12 h. Then, the co-cultures were treated with ROS inhibitors and caspase-1 inhibitors for 30 min before PM2.5 exposure for 4 h. Z-VAD-FMK, Z-YVAD-FMK, NAC, DPI, and MitoQ were included. a Apoptosis of co-cultured neurons were evaluated by flow cytometry with annexin V/PI staining. b Cell viability of co-cultured neurons were assessed via MTT assay. All figures are representative of three independent experiments, performed in triplicate. *P < 0.05 vs. neurons in co-cultures with LPS-primed microglia. #P < 0.05 vs. neurons in co-cultures with oAβ-stimulated microglia. †P < 0.05 vs. neurons in co-cultures with PM2.5-oAβ-stimulated microgliaBack to article page