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Table 3 Characteristics of primary antibodies and staining details

From: Neuroinflammation is increased in the parietal cortex of atypical Alzheimer’s disease

Antibody Antigen Species Origin details Dilution Incubation time Antigen retrieval Detection method
pTau, clone AT8 Tau phosphorylated at Ser202 and Thr205 Mouse IgG1 ThermoFisher, Pittsburgh, USA 1:10000 32 min at 36 °C Heat-induced (pH 8.5) for 24 min Optiview
Amyloid-beta, clone IC-16 N-terminus of amyloid-beta (aa 1-16) Mouse IgG2a Dr. Carsten Korth, University of Dusseldorf, Germany 1:25 32 min at 36 °C Heat-induced (pH 8.5) for 24 min Optiview
GFAP, clone EP672Y Glial fibrillary acidic protein Mouse Roche, Basel, Switzerland 1: 2 8 min at 37 °C Heat-induced (pH 8.5) for 32 min Optiview
Iba1 C-terminus of Iba1 Rabbit Wako Pure Chemical Industries, Osaka, Japan 1:4000 32 min at 36 °C Heat-induced (pH 8.5) for 16 min Optiview
CD68, clone KP1 CD68 Mouse IgG1 Dako, Glostrup, Denmark 1:1200 Overnight at 4°C Heat-induced (pH 6.0) by autoclave EnVision
HLA-DP/DQ/DR, clone CR3/43 Alpha and beta-chains of all products of the DP, DQ, and DR subregions Mouse IgG1 Dako 1:800 Overnight at 4°C Heat-induced (pH 6.0) by autoclave EnVision
C1q C1q Rabbit Dako 1:25600 Overnight at 4°C Heat-induced (pH 6.0) by autoclave EnVision
C3d C3d Rabbit Dako 1:3200 Overnight at 4°C Heat-induced (pH 6.0) by autoclave EnVision
C4b C4b Rabbit Abcam, Cambridge, United Kingdom 1:1600 Overnight at 4°C Heat-induced (pH 6.0) by autoclave EnVision
C5b-9, clone WU13-15 Neoepitope on C9 in the membrane attack complex Mouse Hycult Biotech, Plymouth meeting, USA 1:400 Overnight at 4°C Heat-induced (pH 6.0) by autoclave EnVision