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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Ecto-5′-nucleotidase (CD73) attenuates inflammation after spinal cord injury by promoting macrophages/microglia M2 polarization in mice

Fig. 7

Differentially expressed proteins screened via inflammatory cytokine array. After siRNA or pcDNA3.1 transfection for 24 h, BV2 cells were challenged with IL-4 for another 8 h to activate M2 activation, then the content of inflammatory cytokines in the supernatant fluid of cultured BV2 cells was detected by inflammatory cytokine array. Besides, the inflammatory cytokines in the lesion areas after SCI both from CD73 KO mice and WT mice were included into this detection. The differentially expressed proteins screening criteria were as follows: fluorescence intensity > 1000, fold change > 1.5, and p < 0.05. af Heatmaps of inflammatory cytokines fold changes and representative photographs of inflammatory cytokine array reflected the protein expression in CD73 differentially expressed cells or tissues. The differentially expressed proteins were highlighted with red boxes. g Venn diagram depicting the overlap of differentially expressed proteins identified in three groups. IL-10 and TGF-β were the intersection

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