Fig. 4

Primary cultured microglia from gp91^phox−/− and P47^phox−/− mice showed reduced proconvulsive factor expression after LPS, TNFα, and IL1β stimulation. Microglia-enriched cultures from brains of wild-type (WT), gp91^phox−/−, and P47^phox−/−mice were seeded (7.5 × 10⁴/well) in 24-well plates and were either treated with LPS 5 ng/ml (a), TNFα 500 pg/ml (b), or IL-1β 500 pg/ml (c) or vehicle for 1 h. Microglia were then subjected to RNA preparation and RT-qPCR analyses. β-actin mRNA expression served as the internal control. The expression of each cytokine of the treated cells was compared to that of the genotype-matched vehicle-treated cultures. Data present the mean ± SEM of three independent experiments performed in triplicate. Bonferroni post hoc test vs. corresponding control; *p < 0.05, **p < 0.01, ***p < 0.001