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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: NADPH oxidases as potential pharmacological targets against increased seizure susceptibility after systemic inflammation

Fig. 5

DPI post-treatment attenuated the increased seizure susceptibility following systemic inflammation. a The protocol for DPI post-treatment experiments is schematized. Wild-type (WT) mice were either injected intraperitoneally (i.p.) with 4 mg/kg LPS or with vehicle (saline). Then, the mice were treated with 0, 0.01, 0.1, or 1 mg/kg DPI or vehicle (0.025% DMSO) 30 min and 24 h after LPS injection. 48 h later, all the mice received PTZ (60 mg/kg, i.p.) treatment (n = 6 or 7 mice/group). b Plasma samples were collected 1 h after LPS injection, and the levels of TNFα were determined and compared with that of the vehicle-treated mice. Data present the mean ± SEM; n = 5 for each treatment. Bonferroni post hoc test vs. LPS-vehicle-treated group; *p < 0.05. c The latency to initial seizure onset (clonic with/without tonic convulsion) after PTZ administration. Data are presented as mean ± SEM. Bonferroni post hoc test vs. LPS-injected vehicle-treated group; **p < 0.01. d Seizure susceptibility scored once every 5 min for 2 h after PTZ injection. Two-way repeated measures ANOVA revealed that the main effect for LPS-injectee vehicle-treated and LPS-DPI-treated groups yielded an F ratio of F(3,576) = 18.06, p < 0.0001. Bonferroni test analysis further revealed significant difference between the LPS-injected vehicle-treated group LPS-injected 1 mg/kg DPI-treated group (F(1,288) = 41.05; p < 0.0001). e The total duration (min) of seizure behavior ≥ stage 4. Data present the mean ± SEM. Bonferroni post hoc t-test vs. LPS-injected vehicle treatment; *p < 0.05, **p < 0.01

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