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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: NADPH oxidases as potential pharmacological targets against increased seizure susceptibility after systemic inflammation

Fig. 8

DPI post-treatment attenuated gp91 expression in brain after LPS stimulation in mice. a Wild-type mice (n = 3/group) were treated with LPS and were sacrificed 0, 3, 6, 18, and 24 h after LPS and the brains were harvested. The transcript levels of gp91 were determined by RT-qPCR analyses and compared with that of the vehicle-treated control (to which a value of 1 was assigned). Data present the mean ± SEM. Bonferroni post hoc test vs. LPS-injected vehicle-treated group; **p < 0.01, ***p < 0.001. b The mice were treated with a single dose of vehicle or DPI (0.01, 0.1, or 1 mg/kg) 30 min after LPS injection and then were sacrificed 24 h after LPS. The transcript levels of gp91 were determined by RT-qPCR analyses and compared with that of the LPS-injected vehicle-treated control (to which a value of 1 was assigned). Data present the mean ± SEM. Bonferroni post hoc test vs. LPS-injected vehicle-treated group; *p < 0.05, **p < 0.01. c The brains as described in b were analyzed by western blot analyses to determine the levels of gp91 protein. Representative blots are shown. d The gp91 signals were quantitated and normalized to the internal control. All the normalized values were compared to the LPS-injected vehicle-treated group (to which a value of 1 was assigned). Data represent the mean ± SEM of the results generated from three animals for each group. Bonferroni post hoc test vs. LPS-injected vehicle-treated group; *p < 0.05, **p < 0.01

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