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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Dual modulation on glial cells by tetrahydroxystilbene glucoside protects against dopamine neuronal loss

Fig. 5

TSG enhanced neurotrophic factors release in LPS-induced DA neurotoxicity. Primary neuron-glia cultures were treated with TSG for 30 min followed by LPS addition. The levels of GDNF and BDNF in neuron-glia culture supernatant 1, 4, and 7 days after TSG treatment were measured by ELISA, respectively (a) (GDNF-1 day: the two-way ANOVA interactions analysis of showed F = 0.169 and P = 0.692; GDNF-4 day: the two-way ANOVA interactions analysis of showed F = 0.012 and P = 0.917; GDNF-7 day: the two-way ANOVA interactions analysis of showed F = 0.004 and P = 0.951; BDNF-1 day: the two-way ANOVA interactions analysis of showed F = 0.023 and P = 0.884; BDNF-4 day: the two-way ANOVA interactions analysis of showed F = 0.393 and P = 0.548; BDNF-7 day: the two-way ANOVA interactions analysis of showed F = 0.009 and P = 0.928). The protein levels of BDNF and GDNF in neuron-glia co-culture cells 4 and 7 days after TSG treatment were determined by western blotting. Quantified results were shown as a percentage of the control cultures (b) (GDNF-4 day: the two-way ANOVA interactions analysis of showed F = 0.518 and P = 0.492; GDNF-7 day: the two-way ANOVA interactions analysis of showed F = 0.100 and P = 0.760; BDNF-4 day: the two-way ANOVA interactions analysis of showed F = 1.594 and P = 0.242; BDNF-7 day: the two-way ANOVA interactions analysis of showed F = 0.004 and P = 0.952). Data were the mean ± SEM from three independent experiments performed in triplicate. #P < 0.05 compared with control cultures; *P < 0.05 compared with LPS-treated cultures

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