Fig. 8

TSG modulated glial cells in vivo. Rat brains were sectioned and immunostained with an anti-OX-42 (a) and GFAP (d) antibodies. The images were representative of six rats in each group. Scale bar = 200 μm. The “ellipse” presented the area of SN. The densitometry analysis of SN OX-42-positive microglia (b) (the two-way ANOVA interactions analysis showed F = 4.318 and P = 0.072) and GFAP-positive astroglia (e) (the two-way ANOVA interactions analysis showed F = 0.059 and P = 0.815) from six evenly spaced brain sections from each rat was performed via ImageJ software. In addition, rat midbrains were collected to detect the protein expression of Iba-1 (c) (the two-way ANOVA interactions analysis showed F = 0.049 and P = 0.821) and BDNF and GDNF (f) (BDNF: the two-way ANOVA interactions analysis showed F = 2.928 and P = 0.113; GDNF: the two-way ANOVA interactions analysis showed F = 4.252 and P = 0.079) via western blotting. The ratio of densitometry values of Iba-1, BDNF, GDNF, and β-actin was assessed and normalized to control group. Data were expressed as a percentage of the control group and were the mean ± SEM from six rats. ^#P < 0.05 compared with control group; *P < 0.05 compared with LPS-treated group