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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: RNA sequencing analysis reveals quiescent microglia isolation methods from postnatal mouse brains and limitations of BV2 cells

Fig. 1

Characterization of microglia samples for RNA-Seq. a Scheme showing the workflow for isolation of mouse microglia with three different methods and samples we used for RNA-Seq analysis. b Representative images showing purity and morphology of isolated microglia by immunostaining (Iba1+, green; GFAP+, red). DAPI indicates nuclei. Purple circles highlight amoeboid microglia. Scale bar, 50 μm. c The histogram showing the percentage of amoeboid microglia induced by the three isolation methods. Data are shown as mean + SD, n = 5. One-way ANOVA followed by Tukey’s post hoc test. *P < 0.05

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