Fig. 3

Different isolation methods stimulate microglial activation. Microglia were purified from the mixed glial system using CD11b MACS microbeads, shaking, or mild-trypsin digestion. a Venn diagram analyzed from publicly available microarray data with GEO series ID (GSE) showing similarities and differences of 1180 upregulated genes in microglia treated with LPS or Aβ or during aging. b Heat map and hierarchical clustering of all 1180 upregulated transcripts among microglia isolated by different methods. c Heat map and hierarchical clustering of the common 49 transcripts shared in a among microglia isolated by different methods. The minimal and maximal expression level of each transcript was indicated