Fig. 3
From: Necrotizing enterocolitis is associated with acute brain responses in preterm pigs
Hippocampal transcriptome in pigs with or without NEC. a Venn diagram indicates the number of hippocampal DEGs that were specifically related to Si-NEC (25 genes) and Co-NEC (10 genes), and two genes that were shared for both groups (HBB and TMEM167). These NEC-related DEGs are listed, including the genes further validated by qPCR in a larger cohort (highlighted in light brown, n = 9–16). b Volcano plots of RNA-seq hippocampal gene expression are shown as a scatter-plot of log2 changes in expression of NEC-affected pigs versus No NEC pigs plotted against the negative log of the q value (n = 5–6 per group). The genes up- or downregulated with FDR-adjusted statistical q value < 0.2 are shown as red and blue dots delineated by a dashed line. The transcriptional profiles for Si-NEC and Co-NEC pigs showed overlap in NEC-related effects for two genes, HBB and TMEM 167B (p < 0.0005). c Visualization of enriched biological processes defined by GO terms (yellow triangles), and Si-NEC and Co-NEC DEGs that are involved in the above biological processes are highlighted by red and blue arrows, correspondingly. d qPCR verification for selected NEC-related DEGs and DEGs were identified in biological functions at c by RNA-seq (n = 9–16 per group). Values are means ± SEMs. *p < 0.05; **p < 0.01 vs No NEC pigs (dashed line)