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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Serum amyloid A primes microglia for ATP-dependent interleukin-1β release

Fig. 3

Treatment of rat cortical microglia with Apo-SAA or LPS upregulates in a time-dependent manner mRNA for SAA1. Cultures were treated the day after plating with 0.5 μg/ml recombinant human Apo-SAA or 0.1 µg/ml LPS and processed 1, 3, 6, and 24 h later for q-PCR, as detailed in the “Methods” section. Data are presented as relative expression level (normalized with respect to β-actin (βACT)) at each time point and are mean ± sem, n = 6. Control values (which were at the limit of detection) were omitted for clarity. Apo-SAA (■); LPS (▓). For LPS, *p < 0.05 vs 1 h; for Apo-SAA, °°°p < 0.001 vs 1 h, **p < 0.01 vs 1 h, and ***p < 0.001 vs 6 h

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