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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Serum amyloid A primes microglia for ATP-dependent interleukin-1β release

Fig. 4

Extracellular ATP causes release of IL-1β from rat cortical microglia primed with Apo-SAA or LPS. Cortical microglia cultured in 96-well plates were first incubated 2 h with 0.05–1.5 μg/ml recombinant human Apo-SAA or 0.1 μg/ml LPS. ATP was then added to a final concentration of 5 mM as indicated by upper horizontal bar. After a further 60-min incubation, cell lysates (a) and culture medium (b) were collected for IL-1β analysis by ELISA. Note that Apo-SAA was again a more efficacious priming stimulus than was LPS. Control (CTRL). Apo-SAA (■); LPS (▓). Data are mean ± sem (n = 6). a **p < 0.01 vs LPS only (no ATP). Apo-SAA at all concentrations differed significantly with added ATP (p < 0.001) compared to the same concentration with no ATP. b ***p < 0.001 vs LPS only (no ATP). Apo-SAA at all concentrations differed significantly with added ATP (p < 0.001) compared to the same concentration with no ATP

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