Fig. 5

The P2X₇R antagonist A740003 prevents the ATP-dependent fall in intracellular IL-1β (a) and rise in extracellular IL-1β (b) in Apo-SAA- or LPS-primed rat cortical microglia. Microglia cultured in 96-well plates were pretreated for 30 min with 10 μM A740003 (‘A74’) followed by a 2-h incubation with 0.5 μg/ml recombinant human Apo-SAA (‘SAA’) or 0.1 μg/ml LPS. ATP was then added to a final concentration of 5 mM. After a further 60-min incubation, culture medium (b) and cell lysates (a) were collected for IL-1β analysis by ELISA. Apo-SAA (▓); LPS (▄) Data are mean ± sem (n = 3). ***p < 0.001 vs LPS or LPS + ATP + A740003; °°°p < 0.001 vs Apo-SAA or Apo-SAA + ATP + A740003. There were no statistically significant differences between the LPS/LPS + A740003/LPS + ATP + A740003 and Apo-SAA/Apo-SAA + A740003/Apo-SAA + ATP + A740003 groups