Fig. 8

Effect of the TLR4 antagonist CLI-095 and the TLR1/2 antagonist CU-CPT22 on intracellular IL-1β content in rat cortical microglia treated with LPS, Apo-SAA, or Pam₃CSK₄. The day after plating culture medium was replaced with 50 μl/well of serum-free medium containing 1 μg/ml CLI-095 (‘CLI’) or 20 μM CU-CPT (‘CU’). After 30-min incubation, equal volume of medium was added containing 0.2 μg/ml LPS, 1 μg/ml recombinant human Apo-SAA, or 0.6 μg/ml Pam₃CSK₄. Final concentrations of all added agents were thus one-half of the values indicated. After a further incubation of 3 and 24 h cell lysates were collected for IL-1β analysis by ELISA, as detailed in the “Methods” section. (upper panel) 3 h; (lower panel) 24 h. Data are mean ± sem, n = 4. Control (CTRL; untreated) cultures. *p < 0.05 and ***p < 0.001 vs CTRL; °p < 0.05 and °°°p < 0.001 vs LPS; ^###p < 0.001 vs Apo-SAA; ^#p < 0.05 and vs Pam₃CSK₄