Table 1 PCR primers used in this study
From: Serum amyloid A primes microglia for ATP-dependent interleukin-1β release
Gene target | Direction | Sequence |
|---|---|---|
β-ACT | F | 5′-CCCCATTGAACACGGCATTGTCA-3′ |
R | 5′-ACCCTCATAGATGGGCACAGTGT-3′ | |
IL-1β | F | 5′-TGTGGCAGCTACCTATGTCT-3′ |
R | 5′-GGGAACATCACACACTAGCA-3′ | |
NLRP3 | F | 5′-TGATGCATGCACGTCTAATCTC-3′ |
R | 5′-CAAATCGAGATGCGGGAGAG-3′ | |
SAA1 | F | 5′-ACACGGAGCAGAGGACTCAAG-3′ |
R | 5′-GGTCGAAAGTGGTTGGGGTC-3′ | |
TNF-α | F | 5′-CATCTTCTCAAAACTCGAGTGACAA-3′ |
R | 5′-TGGGAGTAGATAAGGTACAGCCC-3′ | |
TLR2 | F | 5′-TCCATGTCCTGGTTGACTGG-3′ |
R | 5′-AGGAGAAGGGCACAGCAGAC-3′ | |
TLR4 | F | 5′-GATTGCTCAGACATGGCAGTTTC-3′ |
R | 5′-CACTCGAGGTAGGTGTTTCTGCTAA-3′ |