Fig. 4

Activated astrocytes cluster along thrombus-containing brain vasculature. IL-10 KO mice were either infected with P. chabaudi and sacrificed 8 days post-infection or used as uninfected controls. Representative epifluorescence images (× 20) of the hippocampal formation in cryosections (30 μm) from infected (day 8 p.i.) IL-10 KO brains (left, middle) and uninfected IL-10 KO brains (right) immunostained for GFAP (green), fibrinogen (red), and DAPI (blue). IL-10 KO mice were co-stained with WT control samples (n = 5–6 mice per group). Scale bars represent 50 μm