Fig. 6

IL-10 KO mice are rescued from fatal neurologic disease with LMWH treatment. a Two groups of IL-10 KO mice (n = 4) were either treated with 1000 IU/kg (20 IU/dose) enoxaparin Na (ENO) i.p. twice a day (12 h apart) or given saline starting at day 4 post-infection until the middle of the anemic period of disease (day 12 post-infection). b Survival was monitored daily, and blood smears were collected on day 9 post-infection. Behavior was monitored daily using the abbreviated SHIRPA screen (n = 4 mice/group). c Fibrinogen quantification in the brains of untreated and LMWH-treated mice at the peak of infection (day 9 post-infection, n = 4 mice/group). d 30-μm brain hippocampus cryosections stained for astrocytes (GFAP, green). GFAP staining quantified by calculating the percent area per field of immunostaining above signal threshold. One-way ANOVA, followed by post hoc Bonferroni method was used to determine statistical significance. *p < 0.05, **p < 0.01. Scale bars represent 50 μm