Fig. 7

Microglia changes in IL-10 KO mice infected with P. chabaudi. a Representative images of day 8 p.i. WT, IL-10 KO, and LMWH-treated IL-10 KO mice (n = 4 mice/group) 30-μm brain cryosections stained with anti-Iba-1 antibodies and visualized using DAB. b Quantitative analysis of microglia morphology in WT, IL-10 KO, and LMWH-treated IL-10 KO mice using ImageJ software. c Immunofluoresence imaging of microglia (Iba-1-Alexa 488, green), endothelial cells (CD31-Alexa 567, red), and nuclei (DAPI, blue) in 30-μm brain cryosections from WT, IL-10 KO, and LMWH-treated IL-10 KO mice during the peak of infection. Right, 3D reconstruction showing the spatial orientation of microglia cells in relationship to microvasculature in a P. chabaudi-infected IL-10 KO mouse. One-way ANOVA, followed by post hoc Tukey’s test, was used to determine statistical significance. **p < 0.01. Scale bars represent 20 and 50 μm