Fig. 5

Viability of primary murine microglial cells after treatment with a high concentration of activin A. a Comparison of WST-1 assay results of unstimulated primary murine microglial cells and microglial cells after treatment with 13 μg/ml activin A for 48 h (p = 0.69). Data are presented as means + SD of the optical density (OD) at 490 nm (n = 9 from three experiments). b Representative isolectin B4 stainings of unstimulated primary murine microglial cells and microglial cells after treatment with 13 μg/ml activin A for 48 h. By light microscopy, no differences in morphology and density of microglial cells were detected after treatment with activin A