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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Co-aggregation of pro-inflammatory S100A9 with α-synuclein in Parkinson’s disease: ex vivo and in vitro studies

Fig. 2

Optical and fluorescence microscopy of Lewy bodies in the substantia nigra and frontal lobe regions of five PD patients. Representative sequential immunohistochemistry of Lewy bodies formed in neuronal cells in the PD substantia nigra tissues and conducted by using the pair of consecutive S100A9 (a, c) and α-syn (b, d) antibodies, respectively. e Congo red staining of intracellular Lewy body in the substantia nigra. f Immunofluorescence of the representative substantia nigra tissue with α-syn antibodies (shown in orange), S100A9 antibodies (in green), their superposition (in yellow), and DAPI staining of cell nuclei (in blue). The Lewy body in the center of image is shown as a well-defined round-shaped intracellular inclusion. The Lewy body in the lower right corner is shown in yellow indicating staining with both S100A9 and α-syn antibodies. g, h Immunohistochemistry of the representative frontal lobe tissues with α-syn antibodies. i, j Immunofluorescence of the representative frontal lobe tissues with α-syn and S100A9 antibodies and DAPI staining. Color representation as in f. In the images, the labelling notes for S100A9 and α-syn antibody staining is shown in green and orange, respectively. Scale bars are 50 μm in ad, g, and h, 20 μm in e, f, and 100 μm in i, j

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