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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Targeted intracerebral delivery of the anti-inflammatory cytokine IL13 promotes alternative activation of both microglia and macrophages after stroke

Fig. 6

Transplantation of IL13-producing MSCs results in alternative activation of microglia and macrophages at day 14 after ischemic stroke. a Representative fluorescent microscopic images of ischemic cortical region display the expression of F4/80 on CX3CR1eGFP/+ and CCR2RFP/+ cells, acquired from the three experimental groups at day 14 after stroke. Illustrative ×20 close-up magnification represents the changes in F4/80 expression by microglia and macrophages. F4/80 biomarker is expressed on both cell populations in the hemisphere ipsilateral to the lesion. Immunofluorescence colors: blue, TOPRO; green, microglia; red, macrophages; white, F4/80. b Detailed analysis of the expression of biomarker F4/80 revealed a significant increase of F4/80 expression of CCR2RFP/+ macrophages upon IL13-MSCs transplantation in comparison to other two control groups. c The corresponding exploded pie charts show the distribution of F4/80 expression by GFP+ and RFP+ cells in all three experimental groups. In the pie chart, GFP+ microglia and RFP+ macrophages are encircled in green and red, respectively. n = 5–6 mice in each group. Data represent mean ± SD. The data were compared between the three experimental groups using a parametric one-way ANOVA test with Bonferroni’s post hoc test

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