Fig. 4From: In utero electroporation induces cell death and alters embryonic microglia morphology and expression signatures in the developing hypothalamusForeign DNA is responsible for altering microglia morphology and expression signatures in the embryonic brain. Expression of Iba1 and Cd45 in a, k E15.5 wild-type, b, l E15.5 pCIG2 IUE (E14.5), c, m E15.5 brains electroporated with electrode paddles at E14.5, d, n E15.5 brains injected with elution buffer (EB) at E14.5, or e, o E15.5 brains stabbed with a sterile glass needle at E14.5. Expression of Iba1 and P2ry12 in f, p E15.5 wild-type, g, q E15.5 pCIG2 IUE (E14.5), h, r E15.5 brains electroporated with electrode paddles at E14.5, i, s E15.5 brains injected with EB at E14.5, or j, t E15.5 brains stabbed with a sterile glass needle at E14.5. Hypothalamus (a–j) and cortex (k–t). 3V, third ventricle; LV, lateral ventricle. Dashed lines outline the ventricles and mark the division between the thalamus and hypothalamus. White arrows mark Iba1+/Cd45high double-positive (b–d, l–m), or Iba1+/P2ry12+ double-positive (f–j, p–t) cells, while white arrowheads mark Cd45high single-positive (b, l–m), or Iba1+ single-positive (g–j, q–t) cells. Scale bar represents 250 μm (a–t). u Quantification of Iba1+/Cd45high cells within the parenchyma of the hypothalamus in wild-type brains as compared to IUE brains and the other treatments (mean ± SEM; wild-type n = 3; IUE n = 3, p < 0.0001; electrodes n = 3, p = 0.0006; inject EB n = 3, p = 0.002; needle n = 4, p = 0.2194). v Quantification of Iba1+/Cd45high cells within the parenchyma of the cortex in wild-type brains as compared to IUE brains and the other treatments (mean ± SEM; wild-type n = 3; IUE n = 3, p = 0.0023; electrodes n = 3, p = 0.0175; inject EB n = 3, p = 0.1963; needle n = 4, p = 0.2660)Back to article page