Fig. 7

Microglia morphology and expression signatures are comparable to wild-type 3 days following in utero electroporation. a, e E17.5 wild-type Iba1 and Cd45 expression in the hypothalamus (a) and cortex (e) show an even distribution of Iba1+ cells throughout the brain parenchyma. b, f E17.5 pCIG2 IUE (E14.5) Iba1 and Cd45 expression in the hypothalamus (b) and cortex (f) show Iba1+ cells are evenly distributed throughout the brain parenchyma, while very few Cd45^high cells are present in the brain parenchyma. c, g E17.5 wild-type Iba1 and P2ry12 expression in the hypothalamus (c) and cortex (g) show even distribution of both Iba1+ and P2ry12+ cells throughout the brain parenchyma. d, h E17.5 pCIG2 IUE (E14.5) Iba1 and P2ry12 expression in the hypothalamus (d) and cortex (h) show Iba1+ and P2ry12+ cells are relatively evenly distributed throughout the brain parenchyma. Scale bar represents 250 μm (a-e”). 3V, third ventricle. Dashed lines outline the ventricles and mark the division between the thalamus and hypothalamus. White arrows mark Iba1+ single-positive (a–b, e–f) or Iba1+/P2ry12+ double-positive (c–d, g–h) cells, while white arrowheads mark Iba1+/Cd45^high double-positive (b, f) or Iba1+ single-positive (c–d, g–h) cells. i Quantification of Iba1+/Cd45^high cells within the parenchyma of the hypothalamus in IUE brains as compared to wild-type (mean ± SEM; wild-type n = 3, IUE n = 3; p = 0.2227). j Quantification of Iba1+/Cd45^high cells within the parenchyma of the cortex in IUE brains as compared to wild-type (mean ± SEM; wild-type n = 3, IUE n = 3; p = 0.1551)