Table 3 List of primary and secondary antibodies used for western blot analysis
Antibody | Host | Company | Catalog number | Dilution | Incubation conditions |
|---|---|---|---|---|---|
IRAK1 | Rabbit | Abcam | ab238 | 1:1000 | Overnight 4 °C |
TRAF6 | Rabbit | Abcam | ab181622 | 1:1000 | Overnight 4 °C |
pNF-κB (p65) | Mouse | Cell signaling technology | #13346 | 1:1000 | Overnight 4 °C |
β-actin | Mouse | ZSGB-BIO | TA-09 | 1:1000 | Overnight 4 °C |
Anti-rabbit IgG horseradish peroxidase (HRP) | Goat | ZSGB-BIO | ZDR-5306 | 1:3000 | 1Â h RT |
Anti-mouse IgG horseradish peroxidase (HRP) | Goat | ZSGB-BIO | ZDR-5307 | 1:3000 | 1Â h RT |