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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: JNK-mediated microglial DICER degradation potentiates inflammatory responses to induce dopaminergic neuron loss

Fig. 5

TAT-DICER prevents DICER degradation. a Schematic diagram of the TAT-DICER and TAT-scramble (TAT-Scr.) peptides. The letters with the light background indicate the 11 amino acid residues of the HIV transactivator protein (TAT). TAT was fused to the N terminus of the 19 amino acids containing Ser 1456 in mouse DICER. b MS analysis of the DICER immunoprecipitated from microglia pre-incubated with TAT-Scr. or TAT-DICER for 24 h and then with MPP+ for 30 min. The phosphorylated serine residue was shown in lowercase bold letter. (c, d) Upper—representative immunoblots of total cell lysates of microglia (c) or BV2 cells (d) incubated with TAT-Scr. or TAT-DICER for 24 h and then with MPP+ for 12 h. Lower—statistics (n = 4 for c and d). e Representative immunoblots of the immunoprecipitates of total cell lysates of BV2 cells incubated with TAT-Scr. or TAT-DICER for 24 h and then with MPP+ for another 8 h in the presence of MG115 and detected with anti-ubiquitin antibody. Data are mean + SEM. **p < 0.001

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