Fig. 6

Degradation of DICER in microglia potentiates inflammatory responses. a The experimental diagram for b–k. Microglia were incubated with TAT-DICER or TAT- TAT-Scr. for 24 h and then with MPP⁺ and/or for the indicated time. b–e Quantitative PCR analysis of TNF-α (b, n = 4), IL-1β (c, n = 3), iNOS (d, n = 4), and COX-2 (e, n = 4) mRNA levels in microglia treated with MPP⁺ or/and LPS. f ELISA examination of protein levels of TNF-α in the supernatant of microglia treated with MPP⁺ or/and LPS. g–j Quantitative PCR analysis of TNF-α (g, n = 7), IL-1β (h), iNOS (i), and COX-2 (j, n = 8) mRNA levels in microglia incubated with TAT-Scr. or TAT-DICER for 24 h and then with MPP⁺ or/and LPS. k ELISA examination of TNF-α protein levels in the supernatant of microglia incubated with TAT-Scr. or TAT-DICER for 24 h then with MPP⁺ or/and LPS (n = 6). Data are mean + SEM. Comparisons between groups were performed with one-way ANOVA with Tukey’s post hoc test (b–f) or two-way ANOVA with Bonferroni post hoc test (g–k). *p < 0.05, **p < 0.01, ***p < 0.001. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001