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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: JNK-mediated microglial DICER degradation potentiates inflammatory responses to induce dopaminergic neuron loss

Fig. 7

Inhibition of microglial DICER degradation suppresses inflammatory responses and reduces tyrosine hydroxylase-positive neuron loss in the mouse MPTP model. a Left—representative immunoblots of total lysates of microglia sorted from VM of mice 3 days after saline or MPTP injection together with TAT-Scr. or TAT-DICER and probed with the antibody against DICER. Right—statistics (n = 5). b, c Representative images of immunohistofluorescence staining with antibodies against Iba1 (top two, b) and GFAP (bottom two, b) in the ventral tegmental area and tyrosine hydroxylase (c) in the SNpc of mice 7 days after the administration of saline or MPTP with TAT-Scr. or TAT-DICER. In b, the images in the small squares magnified from the area indicated by the red arrows. Scale bar, 200 μM. AOD, average optical densities. d Statistics for b (top two, n = 6 for Ibal 1 and n = 5 for GFAP) and c (bottom, n = 4). e Schematic diagram of the working model. Microglial DICER phosphorylation and degradation by MPP+ amplified the inflammation caused by damaged neurons, leading to enhanced DA neuron loss. Data are mean + SEM. *p < 0.05, **p < 0.001, ##p < 0.01

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