Fig. 5

CCL2 expression in abluminal media (M2) from WT and AD BBB models at the ages of 3 months (n = 5 for WT, n = 6 for AD), 6 months (n = 11 for WT, n = 9 for AD), and 12 months (n = 10 for WT, n = 6 for AD). CCL2 levels were analyzed by the 5-plex Luminex® xMAP® assay (for CCL2, CCL3, CCL4, CCL5, and CXCL10) containing a mixture of beads specific for each chemokine as described in the “Methods” section. Chemokine levels are expressed in picograms per millilitre. The mean is represented by a colored line following the origin of cell types (blue for WT and red for AD cells, respectively). ^*P < 0.05 in M2 of AD BBB models compared to WT BBB models at 12 months by Mann-Whitney’s test