Fig. 3
From: Vitexin reduces epilepsy after hypoxic ischemia in the neonatal brain via inhibition of NKCC1
Vitexin downregulated F-actin expression in RBMECs after OGD. FITC-conjugated phalloidin staining for F-actin+ stress fiber formation demonstrated changes in cytoskeletal assembly following OGD in RBMECs. a Confocal images show co-localization of NKCC1 and F-actin in RBMECs. Scale bar = 10 μm. b Merged views of indicated NKCC1 show complete co-localization (b, left). A side overlap of two peaks was taken as a partial co-localization (b, right). c Pearson’s correlation coefficient is shown in graph (c) from five independent experiments analyzed. d, e The distribution of F-actin+ stress fibers was shown by confocal imaging in Control, OGD, OGD+Bum, and OGD+Vit groups. Arrows indicate actin stress fiber formation. The intensity of F-actin fluorescence was determined in 10 fields/well and divided by the number of cells counterstained with DAPI. Each experimental group consisted of three replicates. Scale bar = 10 μm