Table 1 Antibodies applied for western blot
From: Vitexin reduces epilepsy after hypoxic ischemia in the neonatal brain via inhibition of NKCC1
Antibody | Host | Company | Cat. no. | Dilution | Duration |
|---|---|---|---|---|---|
NKCC1 | Goat | Santa Cruz Biotechnology | Sc-21545 | 1:500 | Overnight 4 °C |
ZO-1 | Rabbit | Invitrogen | RA231621 | 1:100 | Overnight 4 °C |
β-actin | Mouse | Protein tech | HRP-60008 | 1:10000 | Overnight 4 °C |
Anti-goat IgG-HRP | Rabbit | Protein tech | SA00001-4 | 1:1000 | 1Â h RT |
Anti-rabbit IgG-HRP | Goat | PMK Biotech | PMK-014-090 | 1:100000 | 1Â h RT |