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Table 3 Antibodies applied for flow cytometry, immunohistochemistry, and fluorescence staining

From: Vitexin reduces epilepsy after hypoxic ischemia in the neonatal brain via inhibition of NKCC1

Antibody Host Company Cat. no. Dilution Applied Stored
NKCC1 Goat Santa Cruz Sc-21545 1:50 ICC,FCM,IHC Overnight 4 °C
NeuN Mouse Millipore #2742283 1:100 ICC Overnight 4 °C
Phalloidin   Sigma P5282 5 μg/ml ICC 1 h RT
Alexa Fluor® 488 Mouse Cell Signaling Technology #4408 1:250 ICC 1 h RT
Anti-goat IgG-Cy3 Rabbit Protein tech SA00009-4 1:50 ICC 1 h RT
Anti-rabbit IgG-Cy3 Goat Protein tech SA00009-2 1:50 ICC 1 h RT
ZO-1 Rabbit/IgG Invitrogen RA231621 0.25 mg/ml ICC,IF Overnight 4 °C
MPO Rabbit Abcam ab9535 1:100 IHC Overnight 4 °C
CD31 Mouse Thermo Fisher Scientific MA3100 1:50 IF Overnight 4 °C
Anti-rabbit IgG Goat PMK Biotech PMK-014-090 1:100000 IHC 1 h RT
F-actin Mouse Abcam ab205 1:100 ICC Overnight 4 °C
Anti-goat IgG-FITC Rabbit Protein tech SA00003-4 1:50 ICC 1 h RT
DAPI   Beyotime C1002 1:2000 ICC,IF,IHC 1 min RT