Table 3 Antibodies applied for flow cytometry, immunohistochemistry, and fluorescence staining
From: Vitexin reduces epilepsy after hypoxic ischemia in the neonatal brain via inhibition of NKCC1
Antibody | Host | Company | Cat. no. | Dilution | Applied | Stored |
|---|---|---|---|---|---|---|
NKCC1 | Goat | Santa Cruz | Sc-21545 | 1:50 | ICC,FCM,IHC | Overnight 4 °C |
NeuN | Mouse | Millipore | #2742283 | 1:100 | ICC | Overnight 4 °C |
Phalloidin |  | Sigma | P5282 | 5 μg/ml | ICC | 1 h RT |
Alexa Fluor® 488 | Mouse | Cell Signaling Technology | #4408 | 1:250 | ICC | 1 h RT |
Anti-goat IgG-Cy3 | Rabbit | Protein tech | SA00009-4 | 1:50 | ICC | 1Â h RT |
Anti-rabbit IgG-Cy3 | Goat | Protein tech | SA00009-2 | 1:50 | ICC | 1Â h RT |
ZO-1 | Rabbit/IgG | Invitrogen | RA231621 | 0.25 mg/ml | ICC,IF | Overnight 4 °C |
MPO | Rabbit | Abcam | ab9535 | 1:100 | IHC | Overnight 4 °C |
CD31 | Mouse | Thermo Fisher Scientific | MA3100 | 1:50 | IF | Overnight 4 °C |
Anti-rabbit IgG | Goat | PMK Biotech | PMK-014-090 | 1:100000 | IHC | 1Â h RT |
F-actin | Mouse | Abcam | ab205 | 1:100 | ICC | Overnight 4 °C |
Anti-goat IgG-FITC | Rabbit | Protein tech | SA00003-4 | 1:50 | ICC | 1Â h RT |
DAPI | Â | Beyotime | C1002 | 1:2000 | ICC,IF,IHC | 1Â min RT |