Fig. 5

Myeloperoxidase staining of neutrophils in the axotomized SCG 1, 3, and 7 days after axotomy. Immunohistochemical staining of neutrophils with an antibody against MPO showed comparable neutrophil cell counts between WT and Ccr2^−/− SCG 1 and 3 days after injury and a significantly higher number of cells in WT ganglia compared to mutants 7 days after injury. Cell counts were performed using ImageJ at 1, 3, and 7 days post injury (a). The counts represent the sum of cells from three images per section. Representative images of IHC staining in the SCG at 1, 3, and 7 days post axotomy are shown for WT Ax (b, d, f) and Ccr2^−/− Ax (c, e, g). All images were taken at × 25 magnification. Scale bar = 20 μm. n = 5 per group. *p < 0.05. **p < 0.001