Table 1 Primers for the detection of mouse gene products by quantitative real-time PCR

Occludin

F: 5′-CAGGTGAATGGGTCACCGAG-3′

R: 5′-TCAAAAGGCCTCACGGACAT-3′

ZO-1

F: 5′-GAACTTTGACCTCTGCAGCAA-3′

R: 5′-AGAAATCGTGCTGATGTGCC-3′

ZO-2

F: 5′-CAGCTTGTAGTTCTGAGCCG-3′

R: 5′-ATGACGATTGACGTCTCCCC-3′

CLDN1

F: 5′-CCACCATTGGCATGAAGTGC-3′

R: 5′-CTGGCATTGATGGGGGTCAA-3′

CLDN2

F: 5′-ATGCCTTCTTGAGCCTGCTT-3′

R: 5′-AAGGCCTAGGATGTAGCCCA-3′

CLDN3

F: 5′-GCAAGGACTACGTCTGAGGG-3′

R: 5′-ACTGTGTGTCGTCTGTCACC-3′

CLDN8

F: 5′-GGAATGCCAATCCATCACGC-3′

R: 5′-AGGCGTGTAGAGGGAAAGGA-3′

CLDN15

F: 5′-TCCGTGACATCCCTTTTGGG-3′

R: 5′-TTGCCATGGACCGTAGACAC-3′

β-Actin

F: 5′-CTACAATGAGCTGCGTGTGG-3′

R: 5′-AAGGAAGGCTGGAAGAGTGC-3′