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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Bone marrow-derived mesenchymal stem/stromal cells reverse the sensorial diabetic neuropathy via modulation of spinal neuroinflammatory cascades

Fig. 2

Effect of MSC on the morphology of sciatic nerve from mice with diabetic neuropathy. Representative photomicrographs of sciatic nerve cross-sections from non-diabetic mice (panel a, control group), diabetic mice treated with saline (panel c), and diabetic mice treated with MSC (1 × 106, panel e), 12 weeks after the neuropathy induction. Light microscopy revealed that sciatic nerve from diabetic mice (c) had large myelin fibers with axonal atrophy, loose myelin sheath (*), and myelin with infoldings into to the axoplasm (arrow). Panel e shows that sciatic nerve from MSC-treated neuropathic mice presented myelin fibers of various calibers with normal morphology. Scale bar = 40 μm. Electron microscopy of sciatic nerve cross-sections from non-diabetic mice (panel b, control group), diabetic mice treated with saline (panel d), and diabetic mice treated with MSC (1 × 106, panel f). Analysis of ultrastructural aspects of the sciatic nerve shows in b myelin fibers with varying sizes and proportional myelin sheath, including numerous unmyelinated fibers; in d few unmyelinated fibers (arrowhead) and the presence of atrophic axons with loose myelin sheath (*); and in f myelinated fibers with myelin sheath of varying diameters and a large amount of unmyelinated fibers (arrowhead). Scale bar = 2 μm. Panels g and h show the percentage of abnormal myelinic fibers and fibers with myelin infoldings, respectively. Data are expressed as means ± SEM; n = 3 mice per group. *Statistically significant as compared to the control group (p < 0.05). #Statistically significant as compared to the STZ + saline group (p < 0.05). One-way ANOVA followed by Tukey’s multiple comparison test

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