Fig. 2From: Bone marrow-derived mesenchymal stem/stromal cells reverse the sensorial diabetic neuropathy via modulation of spinal neuroinflammatory cascadesEffect of MSC on the morphology of sciatic nerve from mice with diabetic neuropathy. Representative photomicrographs of sciatic nerve cross-sections from non-diabetic mice (panel a, control group), diabetic mice treated with saline (panel c), and diabetic mice treated with MSC (1 × 106, panel e), 12 weeks after the neuropathy induction. Light microscopy revealed that sciatic nerve from diabetic mice (c) had large myelin fibers with axonal atrophy, loose myelin sheath (*), and myelin with infoldings into to the axoplasm (arrow). Panel e shows that sciatic nerve from MSC-treated neuropathic mice presented myelin fibers of various calibers with normal morphology. Scale bar = 40 μm. Electron microscopy of sciatic nerve cross-sections from non-diabetic mice (panel b, control group), diabetic mice treated with saline (panel d), and diabetic mice treated with MSC (1 × 106, panel f). Analysis of ultrastructural aspects of the sciatic nerve shows in b myelin fibers with varying sizes and proportional myelin sheath, including numerous unmyelinated fibers; in d few unmyelinated fibers (arrowhead) and the presence of atrophic axons with loose myelin sheath (*); and in f myelinated fibers with myelin sheath of varying diameters and a large amount of unmyelinated fibers (arrowhead). Scale bar = 2 μm. Panels g and h show the percentage of abnormal myelinic fibers and fibers with myelin infoldings, respectively. Data are expressed as means ± SEM; n = 3 mice per group. *Statistically significant as compared to the control group (p < 0.05). #Statistically significant as compared to the STZ + saline group (p < 0.05). One-way ANOVA followed by Tukey’s multiple comparison testBack to article page