Fig. 5
MSC transplantation reduce glial cell expression in the dorsal horn of the spinal cord of neuropathic mice. Eight weeks after the treatment with MSC (1 × 106; STZ + MSC) or saline (STZ + saline), glial cell expression in the spinal cord of neuropathic mice was evaluated. Control non-diabetic group received saline instead of streptozotocin. Representative photomicrographs of histological sections of the mouse spinal cord immunolabeled with GFAP (a–c) or Iba1 (e–g). Images are at 200×. Insets demonstrate representative photomicrographs of GFAP or Iba1 positive cells under magnification of 400×. Scale bar = 50 μm. Panels d and h show the quantitative analysis of the percentage area GFAP and Iba1 positive in the spinal dorsal horn, respectively. Data are expressed as means ± SEM; n = 3 mice per group. *Statistical significance compared to the remaining groups (p < 0.05). One-way ANOVA followed by Tukey post-test